GC Image Users' Guide
Answers to Frequently Asked Questions (FAQ)
This is a list of Frequently Asked Questions (FAQ) about GC Image
software. The answers are linked below.
This page is currently under construction. If you have questions
about GC Image not listed on this page, please forward them to us.
Questions about GC Image software.
Questions about installing, starting, exiting, and uninstalling GC Image.
Questions about input and output of GCxGC files in GC Image.
Questions about viewing GCxGC images in GC Image.
Questions about colorizing GCxGC images in GC Image.
Questions about image data visualization tools in GC Image.
Questions about graphics in GC Image.
Questions about image processing in GC Image.
Questions about blob detection in GC Image.
Questions about blob statistics and metadata in GC Image.
Questions about pattern recognition in GC Image.
Questions about configuration files and operations journals.
Questions about mass spectrum data in GC Image.
Questions about project management and reporting in GC Project.
Questions about project files and directories in GC Project.
Questions about vials in GC Project.
Questions about runs in GC Project.
Questions about processing files in GC Project.
Questions about processed images in GC Project.
Questions about the hierarchical overview in GC Project.
Questions about batches in GC Project.
Questions about reports in GC Project.
This is a list of Answers to Frequently Asked Questions (FAQ) about
GC Image software.
- What is GC Image?
GC Image is software for visualizing, processing, analyzing, and
reporting on images produced in two-dimensional gas chromatography
(GCxGC).
GC Image was created by Dr. Stephen E. Reichenbach and others in the
Computer Science and Engineering Department at the University of
Nebraska - Lincoln and now is being developed by GC Image, LLC.
For technical information about the GC Image software, contact
GC Image, LLC
PO Box 57403
Lincoln NE 68505-7403 USA
Phone: 1.402.310.4503
Fax: 1.402.436.2660
Email:
info@gcimage.com
GC Image is proprietary software, with copyrights and trademarks held
by GC Image, LLC, and the University of Nebraska - Lincoln.
- How do I obtain GC Image software?
For information about licensing the GC Image software, contact:
Zoex Corporation
Lincoln NE USA
Phone: 1.402.475.7640
Email: info@zoex.com
- How do I view GC Image version and license information?
Step 1: Select About GC Image from the Help menu of
the Image Viewer.
The About GC Image pop-up, with version and license information,
is displayed.
- How do I view the GC Image Users' Guide?
Step 1: Select Users' Guide from the Help menu of
the Image Viewer.
GC Image launches Microsoft Internet Explorer which displays the
HTML-formatted GC Image Users' Guide.
Answers to questions about installing, starting, exiting, and uninstalling GC Image.
- What computer configuration is required for GC Image?
GC Image software, Version 1.4 (February 2004), is distributed for
installation on computer systems running a recent Microsoft Windows
operating system, e.g., Windows NT (Service Pack 5 or later),
Windows 2000, or Windows XP.
The suggested minimum hardware configuration is a 1000MHz Intel Pentium 4
(or comparable) CPU, 512MB RAM (1024MB RAM is preferred, especially for
GCxGC-MS), 32MB graphics card (64MB is preferred) and color monitor with
1024x768 (or higher) resolution.
GC Image software requires relatively little hard-disk space
(about 150MB), but images processed by the system (including the
example images distributed with the software) may require large
disk capacity.
- How do I install GC Image software?
Step 1: Execute setup.exe, a self-extracting program
that installs the software. To install GC Image, the user must agree
to the software license terms and conditions.
Step 2: Specify the folder location for the software.
The setup program adds a link to GC Image in the Start->Programs
list and places a shortcut on the desktop.
Step 3: Follow the instructions for the NIST MS Search Demonstration
software (if desired for GCxGC-MS data).
Step 4: Use the license number to obtain the license key from
Zoex Corporation. Then, enter the license key in license popup.
- How do I start GC Image?
Step 1: Execute GC Image from the Start->Programs, from
the user-specified installation folder, or from the shortcut on the
desk-top.
- How do I set the license key in GC Image?
Note: This step is required only the first time the software is
run after installation.
Step 1: Provide the license number to Zoex Corporation and get
license key number.
Step 2: Enter the license key into the License dialog,
then click OK.
- How do I login to GC Image?
Step 1: Enter the username and password in the Login
dialog, then click OK.
- How do add a user for GC Image?
Step 1: Click Add New User on the Login
dialog.
Step 2: Enter the name, username, password (twice), and secret
question and answer, then click OK.
- How do I retrieve a lost password?
Step 1: Click Forgot Password on the Login
dialog.
Step 2: Enter the username on the Forgot Password dialog,
then click OK to be shown the secret question.
Step 3: Enter the answer to the secret question and click
Get Password. If the secret answer is correct, a popup will
give the password.
- How do I exit GC Image?
Step 1: Select Exit from the File menu of the
Image Viewer.
When Exit is selected, the currently open image is discarded.
If an image is open and has been altered since the Save or
Save As operation for that image, GC Image prompts the user
whether or not the image is to be saved before exiting. It is best
to save changed images that are to be retained when they are changed
to prevent the loss of unsaved work (e.g., in the event of a
system crash or power failure) and before requesting the
program to exit.
- How do I uninstall GC Image?
Step 1: Execute the uninstall program from the user-specified
installation folder.
Answers to questions about input and output of GCxGC files in GC Image.
- What are the GCxGC file structures in GC Image?
GC Image represents GCxGC images in two files:
-
A file with .gci extension stores ancillary data about the image (e.g.,
its size, color mapping, etc.) in plain text format tagged using an
eXtensible Markup Language (XML) schema for GCxGC images.
-
A file with .bin extension stores the binary image data in time order
with each value stored as an IEEE double-precision, floating-point
number with big-endian byte order.
- What data formats can be imported by GC Image?
GC Image can import images in a variety of file formats.
| Extension |
Format |
| .asc |
ASC - Thermo ASCII XY text format. Currently, metadata values
are read for "Sampling Rate" and "Y Axis Multiplier". |
| .bin |
Binary - big-endian, IEEE, single-precision, floating-point;
optional byte-swapping supports little-endian format. |
| .bmp |
Bitmap - common Microsoft Windows format. |
| .cdf |
NetCDF - Analytical Data Interchange (ANDI) Protocol for Mass
Spectrometric Data, based on the Unidata/UCAR network Common Data
Format. Currently, not all ANDI metadata values are read. |
| .ch |
CH - Agilent Chemstation IQ Data File Format. Currently, not
all metadata values are read. |
| .csv |
CSV - Comma-Separated-Values in text format;
each line is "<time>, <value>" or each line
has "<value>" only, without time or comma. |
| .gif |
GIF - Graphics Interchange Format. |
| .jpg |
JPEG - Joint Photographic Experts Group format. |
| .png |
PNG - Portable Network Graphics format. |
| .tif |
TIFF - Tagged Image File Format. |
| .txt |
Text format; each line ends with a value, possibly preceded
by other text separated by a tab, colon, semi-colon, comma, or
space. |
- How do I import GCxGC data?
Step 1: Select Import Image from the File menu
of the Image Viewer (or the Import Image button from
the Image Viewer tool bar).
Step 2:
GC Image presents a pop-up dialog box for specifying the source and
destination folders and filenames.
Specify the source and destination folders and filenames,
either by typing in the text boxes or by using the file-system browsers.
The determination as to the input image file type is made on the basis
of the file name extension, so files to be imported should be named
with the proper extension. For more information on importing images,
see chapter Image Input and Output.
Step 3: For file formats, such as CSV, that do not supply the
dimensions of the image, enter size parameters in the pop-up dialog
box for image dimensions.
Step 4: If desired, choose to apply a specified processing
configuration file and indicate the file.
Step 5: If desired, designate optional phase shift, optional
background removal, optional blob detection (if background removal
is performed), optional template matching with a specified template
file (if blob detection is performed) with a specified template,
optional saving of the blob table to a specified file (if blob
detection is performed), optional saving of the image to a specified
file, and optional exiting.
- How do I open an image?
Step 1: Select Open Image from the File menu
of the Image Viewer (or the Open Image button from the
Image Viewer tool bar).
GC Image launches a file-system browser to locate the folder and
.gci file.
Step 2: Specify the folder and filename in the file-system browser.
- How do I open a recently used image?
Step 1: Select an image from the sub-menu list for the Recent
Images item on the File menu of the Image Viewer.
- How do I save an image?
Step 1: Select Save from the File menu of the
Image Viewer (or the Save button from the Image Viewer
tool bar).
- How do I save an image with a different file name?
Step 1: Select Save As from the File menu of the
Image Viewer.
GC Image launches a file-system browser to specify the folder and
GCI .gci filename.
Step 2: Specify the folder and filename in the file-system browser.
- How do I export an image?
Step 1: Select Export Image from the File menu
of the Image Viewer.
GC Image launches a file-system browser to specify the folder and
GCI .gci filename.
Step 2: Specify the folder and filename in the file-system browser.
Also, choose to attach or not to attach axes to the exported image.
- How do I print an image?
Step 1: Select Print Image from the File menu
of the Image Viewer.
GC Image launches a print preview popup to specify the printing layout.
Step 2: Specify the printing layout and click the Print button.
- How do I close an image?
Step 1: Select Close from the File menu of the
Image Viewer (or the Close button from the Image
Viewer tool bar).
When Close is selected, the current image is discarded. If the
current image has been altered since the Save or Save As
operation for that image, GC Image prompts the user whether or not the
image is to be saved before closing. It is best to save all changed
images that are to be retained when they are changed to prevent the loss
of unsaved work (e.g., in the event of a system crash or power
failure) and before requesting the image be closed. This menu
option is not available if no image is open.
Answers to questions about viewing GCxGC images in GC Image.
- What is the Image Viewer?
The Image Viewer is the main interface of GC Image.
The Image Viewer has (from top to bottom):
- title bar, which just displays the name of the current image;
- menu bar, which provides menu access to all operations, tools,
settings, and help resources;
- tool bars, with buttons for commonly used operations, tools, and
cursor modes;
- Viewport, which displays the region-of-interest of the
current image with axes; and
- status bar, which displays the position and value of the image
pixel at the cursor location.
- How do I resize the Image Viewer?
Step 1: With the cursor over a border or corner of the Image
Viewer window, click-and-drag the border or corner to the desired
size.
(The Maximize button on the title bar makes the Image Viewer
fill the screen and the Restore down button will restore the
previous size after maximization.)
- How do I navigate (i.e., scroll and pan) about an image using the Image Viewer?
Step 1: Select View mode from the Image Viewer
cursor-mode palette.
Step 2: With the cursor in the Viewport, click-and-drag
the image as desired.
(Also, after clicking in the image, use the arrow, page-up, and page-down
keys.)
- How do I scale (or zoom) an image using the Image Viewer?
Step 1: Select a value from the pull-down menu of scale factors
or type a value in the textbox in the Scale Viewport tool on the
Image Viewer tool bar.
- How do I resize an image?
Step 1: Select Resize from the View menu of the
Image Viewer.
GC Image launches a pop-up dialog box for the desired dimensions.
Step 2: Specify the desired values, then click OK.
- How do I simultaneously scale and resize a region of interest?
Step 1: Select View mode from the Image Viewer
cursor-mode palette.
Step 2: With the cursor in the Viewport, click-and-drag
with the right-mouse button to delimit the desired region of interest.
- How do I reset the scale and size of the displayed image?
Step 1: Click the Reset Display button on the Image
Viewer tool bar.
- What is the Navigator?
The Navigator provides a "thumbnail" view of the entire
image (typically at reduced resolution).
The Navigator also provides an interface to visualize and relocate
the region-of-interest (ROI) that is displayed in the Viewport
of the Image Viewer.
The Navigator can be set to maintain aspect ratio or to fit the
image to the window.
Navigator can be launched by the too bar button or from the
View menu of the Image Viewer.
- How do I navigate (i.e., scroll and pan) about an image using the Navigator?
Step 1: With the cursor in the ROI box of the Navigator,
click-and-drag the ROI to the position desired. More precise
positioning can be specified via the Set Position button
on the Navigator tool bar.
- How do I scale (or zoom) an image using the Navigator?
Step 1: With the cursor positioned on a corner of the ROI box of
the Navigator, click-and-drag the corner of the ROI to the size
desired.
- How do I change the units on the Viewport axes?
Step 1: Select Configure Settings from the Configure
menu of the Image Viewer.
GC Image launches a pop-up with various configuration dialog panes.
Step 2: Select the Axes configuration pane.
Step 3: Select the desired units for the axes (e.g.., time
or pixels).
Click the OK button.
Answers to questions about colorizing GCxGC images in GC Image.
- What is colorization?
Colorization maps pixel values to colors for display.
Within the user-configurable Value Range, values are mapped
through a Value Map that applies a logarithmic, linear, or
exponential function.
Then, the value-mapped values are mapped through three Color Maps,
using either Red, Green, and Blue (RGB) or Hue, Saturation, and Value
(HSV or sometimes HSB for Brightness) Color Space.
The Color Maps are defined using multiple knots and piecewise
linear or cubic spline interpolation.
The three color maps define a wheel that can be rotated with a Color
Shift.
Values outside the Value Range are set to Extrema Colors.
Open the Colorize tool by either clicking the Colorize button
on the Image Viewer tool bar or selecting View->Colorize on
the Image Viewer menu bar.
- How do I set the value range for colorization?
Step 1: Enter the minimum (Min) and/or maximum (Max) values
defining the value range in the text boxes in the upper-right corner
of Colorize. Type the ENTER or RETURN key on the keyboard to
enter the values.
Step 2: To apply the value range to the image, click the Apply
button or the OK button.
- How do I set the Extrema Colors for values outside the value range?
Step 1: Select either automatically assigned (Auto) or user-specified
(Custom) Extrema Colors. (Automatically assigned Extrema Colors
are set to the colors of the minimum and maximum values.)
Step 2: For Custom Extrema Colors, click the left color box
to set the color for values less than the minimum and the right color box
to set the values greater than the maximum value. GC Image launches a
color chooser.
Step 3: In the color chooser, set the desired color using swatches,
HSB sliders, or RGB sliders, then click the OK button.
Step 4: To apply the Extrema Colors to the image, click the
Apply button or the OK button.
- How do I set Value Mapping for values within the value range?
Step 1: Slide the Value Mapping slider left for logarithmic
value mapping or right for exponential value mapping or click the Value
Mapping button for linear value mapping.
Step 2: To apply the Value Mapping to the image, click the
Apply button or the OK button.
- How do I set Color Mapping for values within the value range?
Step 1: Select either RGB or HSV Color Space.
Step 2: Define the three color mapping functions by clicking to
add to or reduce the number of knots, click-and-drag the knots to the
desired positions, and choosing linear or spline interpolation through
the knots.
Step 3: To apply the Color Mapping to the image, click the
Apply button or the OK button.
- How do I export Colorize settings?
Step 1: Enter a name for the color map in the Import/Export
Colormap textbox.
Step 2: Click the Export button.
- How do I import Colorize settings?
Step 1: Select a color map from the color map list.
Step 2: Click the Import button.
Answers to questions about image data visualization tools in GC Image.
- What image data visualization tools are available in GC Image?
GC Image provides several tools for visualizing image data.
-
Multi-Projections graphs one-dimensional slices and projections
of an image.
-
Text View provides a tabular view of the image data values.
-
3D View tool provides a three-dimensional perspective view of
the image data values.
-
Visualize Data provides pixel statistics and plots statistical
relationships for an image or sub-image.
All of these tools are accessible from the View menu of the
Image Viewer.
- How do I view a one-dimensional slice of an image?
Step 1: In the Multi-Projections control pane, select
integrated projection mode.
Step 2: In the Multi-Projections image pane, click on a
pixel of the desired row and column. GC Image graphs the row slice at
the bottom of Multi-Projections and the column slice at the right
of Multi-Projections.
- How do I view a one-dimensional projection of an image?
Step 1: In the Multi-Projections control pane, select
integrated projection mode.
Step 2: In the Multi-Projections image pane, click-and-drag
from one side of the desired row and column range to the other side.
GC Image graphs the row projection at the bottom of Multi-Projections
and the column projection at the right of Multi-Projections.
- How do I view a multiple one-dimensional slices of an image?
Step 1: In the Multi-Projections control pane, select
multiple slices (Multi) projection mode.
Step 2: In the Multi-Projections image pane, click-and-drag
from one side of the desired row and column range to the other side.
Step 3: In the Multi-Projections image pane, set the number
of horizontal and vertical slices with the sliders or enter values in the
text boxes and hit the RETURN or ENTER.
GC Image graphs the row slices at the bottom of Multi-Projections
and the column slices at the right of Multi-Projections.
- How do I save one-dimensional slices and projections of an image?
Step 1: At the bottom of the Multi-Projections control pane,
click either the horizontal or vertical save buttons. GC Image launches a
file-system browser.
Step 2: Specify the file location and name for the CSV file.
- How do I view the data values in a text table?
Step 1: If desired, select a rectangular sub-region of the image,
using the graphics tool for drawing rectangles, to see the values in
the rectangle. Or, select a blob to see the values in the bounding box
of the blob.
Step 2: Select Text View from the View menu of the
Image Viewer or click the Text View button on the Image
Viewer tool bar. GC Image launches a pop-up tool with the text table
of values.
- How do I export the data values from a text table?
Step 1: Click the Save text view button on the Text View
popup. GC Image launches a file-system browser to specify the folder and
CSV filename.
Step 2: Specify the file location and name, then click Save.
- How do I view the data values in a three-dimensional perspective view?
Step 1: If desired, select a rectangular sub-region of the image,
using the graphic tool for drawing rectangles, to see the values in
the rectangle.
Step 2: Select 3D View from the View menu of the
Image Viewer or click the 3D View button on the Image
Viewer tool bar. GC Image launches a pop-up tool with the three-dimensional
perspective view.
Step 3: Reposition the image using the latitude, longitude, and
distance sliders on the 3D View control panel or with mouse
actions:
-
Translate by click-and-drag with the right mouse-button.
-
Rotate by click-and-drag with the left mouse-button.
-
Zoom (or scale) by click-and-drag with the middle mouse-button.
Currently, translation is available only through the mouse action.
- How do I configure the three-dimensional perspective view?
Step 1: On the 3D View control panel, select the surface
as wireframe or continuous.
Step 2: Select the color as monochromatic or colorized.
For a monochromatic display, select the color. For a colorized
display, set the image color map.
Step 3: Select the initial resolution. Resolutions larger than
1.0 (the default) for large images may require more memory than is
available and cause the program to fail.
Step 4: Click the Apply Above Settings button.
- How do I visualize pixel statistics?
Step 1: If desired, select a rectangular sub-region of the image
using the graphic tool for drawing rectangles.
Step 2: Select Visualize Data from the View menu
of the Image Viewer.
GC Image launches Visualize Data.
Step 3: Select the statistics for the X and Y axes.
Step 4: If desired, click the Histogram button.
GC Image launches Histogram tool.
Answers to questions about graphics in GC Image.
- What graphics are available in GC Image?
GC Image supports point, polyline, rectangle, and polygon graphics.
The Image Viewer palette contains a button to set the cursor
mode to graphics and a pull-down graphics mode selector.
The selectable graphic cursor modes are:
- Select Graphic - Select a graphic.
- Draw Point - Draw a point.
- Draw Polyline - Draw a polyline.
- Draw Rectangle - Draw a rectangle.
- Draw Polygon - Draw a polygon.
- How do I draw and edit a point?
Step 1: Set the cursor mode to draw a point mode from the
graphics cursor mode selector on the Image Viewer palette.
Step 2: Click at the desired location. After the click, GC Image
will revert to select a graphic mode with the drawn point selected.
Step 3: Click-and-drag on the point to move the graphic.
- How do I draw and edit a polyline?
Step 1: Set the cursor mode to draw a polyline mode from the
graphics cursor mode selector on the Image Viewer palette.
Step 2: Click the left mouse-button at each knot of the desired
polyline, except double click at the final knot to end the polyline.
After the polyline is ended, GC Image will revert to select a graphic
mode with the drawn polyline selected.
Step 3: Click-and-drag any knot handle(s) of the polyline to
adjust points. Click-and-drag on the polyline to move the graphic.
- How do I draw and edit a rectangle?
Step 1: Set the cursor mode to draw a rectangle mode from the
graphics cursor mode selector on the Image Viewer palette.
Step 2: Click-and-drag from one corner of the desired rectangle
to the opposite corner of the desired rectangle. After the mouse button
is released, GC Image will revert to select a graphic mode with the
drawn rectangle selected.
Step 3: Click-and-drag any corner handle(s) of the rectangle to
adjust size. Click-and-drag from the interior of the rectangle to move
the graphic.
- How do I draw and edit a polygon?
Step 1: Set the cursor mode to draw a polygon mode from the
graphics cursor mode selector on the Image Viewer palette.
Step 2: Click the left mouse-button at each vertex of the desired
polygon, except double click at the final vertex to close the polygon.
After the polygon is closed, GC Image will revert to select a graphic
mode with the drawn polygon selected.
Step 3: Click-and-drag any corner handle(s) of the polygon to
adjust shape and size. Click-and-drag from the interior of the polygon
to move the graphic.
- How do I configure graphics display settings?
Step 1: Select Configure Settings from the Configure
menu of the Image Viewer.
GC Image launches a pop-up with various configuration dialog panes.
Step 2: Select the Graphics Display configuration pane.
Step 3: Set whether to show graphics in all states or only in
graphics cursor mode, set the color for drawing graphics, then
click the OK button.
- How do I select a graphic?
Step 1: Set the cursor mode to select a graphic mode from the
graphics cursor mode selector.
Step 2: Click on the existing graphic to be selected.
Select additional graphics for the selection set by holding the control
key during the click of the left mouse-button while the cursor is on the
next graphic to be selected. GC Image will place handles on the selected
graphic(s).
- How do I select all graphics?
Step 1: Set the cursor mode to select a graphic mode from the
graphics cursor mode selector.
Step 2: Select Select All item from the Edit menu.
GC Image will select all graphics.
- How do I set and edit graphic(s) metadata?
Step 1: Select one or more graphics using the select graphics
cursor mode.
Step 2: Click the right mouse button. Depending on how many graphics
are selected, GC Image launches either a pop-up metadata dialog for a single
graphic or a pop-up metadata dialog for multiple graphics.
Step 3: For a single graphic metadata, set the desired name,
description, inclusion flag, and color.
For multiple graphics metadata, set the desired description, inclusion
flag, and color.
- How do I delete a graphic?
Step 1: Select the existing graphic(s) to be deleted.
Step 2: Click the Delete / Exclude Object button.
Confirmation is required.
- How do I export graphics?
Step 1: Select Export Graphics from the File menu
of the Image Viewer. GC Image presents a pop-up dialog box for
specifying the destination file.
Step 2: Specify the destination file, then click Save.
- How do I import graphics?
Step 1: Select Export Graphics from the File menu
of the Image Viewer.
Step 2: If graphics are present, GC Image prompts the user
to overwrite or append to the existing graphics. Then, GC Image
presents a pop-up dialog box for specifying the source file.
Step 2: Specify the source file, then click Open.
Answers to questions about image processing in GC Image.
- What image processing operations are available in GC Image?
GC Image has three image processing operations.
-
Shift Phase shifts the phase of the image with respect to the
start time of the second dimension.
-
Correct Baseline removes the slowly varying baseline (or background)
level introduced by the system.
-
Arithmetic Operations apply point-wise addition, subtraction,
or multiplication with a scalar value or another image.
These filters are available from the Filters menu on the Image
Viewer menu bar or from buttons on the Image Viewer tool bar.
- How do I shift the second-column phase of an image?
Step 1: Select Shift Phase from the Filters menu
on the Image Viewer menu bar or click the Shift Phase
button on the Image Viewer tool bar.
GC Image launches a pop-up dialog box.
Step 2: Specify the desired phase shift (a positive value for
upward shift and a negative value for downward shift) in the textbox,
then click the OK button.
- How do I reset shift of the second-column phase of an image?
Step 1: Select Shift Phase from the Filters menu
on the Image Viewer menu bar or click the Shift Phase
button on the Image Viewer tool bar.
GC Image launches a pop-up dialog box.
Step 2: Click the Reset button.
- How do I correct the baseline offset of an image?
Step 1: Select Correct Baseline from the Filters menu
on the Image Viewer menu bar or click the Correct Baseline
button on the Image Viewer tool bar.
GC Image applies the baseline correction algorithm, modifying the current
image.
- How do I configure the baseline correction process?
Step 1: Select Configure Settings from the Configure
menu of the Image Viewer.
GC Image launches a pop-up with various configuration dialog panes.
Step 2: Select the Baseline Correction configuration pane.
Step 3: Set the number of deadband pixels, filter window size,
distribution, and stride parameters.
Click the OK button.
See chapter Image Processing for a
description of the baseline correction algorithm and parameters.
- How do I perform point-wise arithmetic operations?
Step 1: Select Arithmetic Operations from the Filters
menu in the Image Viewer.
Step 2: From the Arithmetic Operations popup dialog,
select the arithmetic operation, whether the operation is with
a scalar or image operand, and, for an image operand, the operand
file name. Then, click OK.
Answers to questions about blob detection in GC Image.
- What is blob detection?
Blob detection is the process of identifying the pixels that make up
each peak.
Once the blobs of pixels are detected, they can be visualized and
analyzed.
The position (i.e., the retention times in each dimension) of
each blob is related to the physical properties the chemical that produced
the blob, so the position of a blob is useful in identifying each chemical
in a sample.
The sum of the pixel values in each blob is related to the quantity of
the chemical that produced it, so the sum of pixel values of a blob is
useful in quantifying each chemical in a sample.
- How do I detect blobs in an image?
Step 1: Select Detect Blobs from the Filters
menu on the Image Viewer menu bar or click the Blob
Detection button on the Image Viewer tool bar.
GC Image detects the blobs and creates a blob table that can be
analyzed and reported.
The blobs are highlighted graphically in the Viewport of the
Image Viewer and in other tools according to blob display
configuration.
- How do I configure blob detection?
Step 1: Select Configure Settings from the Configure
menu of the Image Viewer.
GC Image launches a pop-up with various configuration dialog panes.
Step 2: Select the Blob Detection configuration pane.
Step 3: Set the number of detection algorithm, smoothing window,
distribution, and filter parameters.
Click the OK button.
See chapter Image Processing for a
description of the blob detection algorithm and parameters.
- How do I configure blob display settings?
Step 1: Select Configure Settings from the Configure
menu of the Image Viewer.
GC Image launches a pop-up with various configuration dialog panes.
Step 2: Select the Blob Display configuration pane.
Step 3: Set what blobs to show, how to show blobs, whether to
show internal standard associations, and colors.
Click the OK button.
- How do I select blob(s)?
Step 1: Set the cursor mode to one of the blob selection
modes (point, line, rectangle, or polygon) from the graphics cursor
mode selector on the Image Viewers palette.
Step 2: Select blob(s) depending on the blob selection mode.
-
In pixel blob-selection mode, move the cursor inside the border of
a blob and click the left mouse-button.
Select additional blobs for the selection set by holding the control key
during the click of the left mouse-button while the cursor is inside the
next blob to be selected.
-
In line blob-selection mode, move the cursor to one side of a series
of blobs, depress the left mouse-button, move the cursor to the other
side of the series (defining a line through the series), and release
the mouse button. All blobs crossed by the line will be selected.
-
In rectangle blob-selection mode, move the cursor to a point above
(or below) and left (or right) of all blobs to be selected, depress
the left mouse-button, move the cursor below (or above) and right
(or left) of all the blob peaks to be selected (defining a bounding
box), and release the mouse button.
All blobs with the peak pixel in the rectangle will be selected.
-
In polygon blob-selection mode, move the cursor to a point near the
blobs to be selected, click the left mouse-button, repeatedly move
the cursor around the blobs to be selected and click the left
mouse-button (drawing a polygon), and double click the left
mouse-button (to close the polygon).
All blobs with the peak pixel in the polygon will be selected.
GC Image graphically highlights selected blobs in the Viewport
of the Image Viewer according to blob display configuration.
- How do I select all blobs?
Step 1: Set the cursor mode to select blob mode.
Step 2: Select the Select All item from the Edit
menu. GC Image will select all blobs.
- How do I edit blobs with the Edit Blobs tool?
Step 1: Select a focal blob using the blob selection cursor mode.
Step 2: Select Edit Blobs from the Edit menu on
the Image Viewer menu bar or the Edit Blobs button on
the Image Viewer tool bar.
GC Image launches Edit Blobs.
Step 3: Navigate the cross-hairs over the blob (or region) to
be edited by click-and-drag in the image pane of Edit Blobs,
Shift-Click in the image pane of Edit Blobs, or clicking the
arrow navigation buttons.
Step 4: Use the operation buttons to split the blob vertically,
split the blob horizontally, merge the blob with an adjacent blob,
exclude the blob, or add a new blob.
- How do I exclude blobs in the Image Viewer?
Step 1: Select the blob(s) to be excluded.
Step 2: Click the Delete / Exclude Object button.
Confirmation is required.
- How do I draw blobs in the Image Viewer?
Step 1: Select the Draw Blob cursor mode.
Step 2: Click down on the mouse left-button, draw the cursor
around the border of the blob (without crossing the line drawn or other
blobs), and release the button to close the shape.
Answers to questions about blob statistics and metadata in GC Image.
- What blob statistics and metadata are available in GC Image?
GC Image supports more than 80 statistical features and metadata for
blobs.
See the white paper, GCxGC Blob Metadata and
Statistics in GC Image, for details of each of the available
metadata and computed attributes in GC Image.
A few basic of the statistical features computed by GC Image are:
- peak indices - the indices of the largest valued sample in the blob,
- peak value - the largest value in the blob,
- area - the number of pixels in the blob, and
- volume - the sum of pixel values in the blob.
Some of the metadata features which can be set by the user in GC Image are:
- compound name - the name of the chemical compound associated with the
blob,
- group name - the name of a chemical group to which the chemical compound
associated with the blob belongs,
- inclusion flag - whether the blob is identified for inclusion in
reporting,
- exclusion flag - whether the blob is identified for exclusion from
all computations in reporting,
- internal standard - whether the chemical compound associated with the
blob is an internal standard.
- How do I set and edit metadata for blob(s)?
Step 1: Select one or more blobs using the select blobs cursor
mode.
Step 2: Click the right mouse button. Depending on how many blobs
are selected, GC Image launches either a pop-up metadata dialog for a single
blob or a pop-up metadata dialog for multiple blobs.
Step 3: For a single blob metadata, set the desired compound
name, group name, constellation name, inclusion flag, internal standard
flag, and exclusion flag.
For multiple blobs metadata, set the desired group name, constellation
name, inclusion flag, internal standard flag, and exclusion flag.
- How do I cut, copy, and paste metadata for blob(s)?
Step 1: Select a blob(s) using the blob selection cursor.
(Metadata Cut will work for multiple blobs, but Copy Metadata
and Paste Metadata will not for multiple blobs.)
Step 2: Select Cut Metadata, Copy Metadata, or
Paste Metadata from the Edit menu of the Image Viewer
or click the appropriate button on the Image Viewer bar.
GC Image modifies the blob table accordingly.
- How do I view the blob table?
Step 1: Select Blob Table from the View menu
of the Image Viewer or click the Blob Table button
on the Image Viewer tool bar.
GC Image launches Blob Table viewer.
Step 2: Use the scroll bars to navigate the blob table.
Sort the table by clicking on a column header (and click again
to reverse order).
- How do I select blobs from the blob table?
Step 1: Bring up the blob table.
Step 2: Click on a row of the blob table selects the blob.
Click and click again with the shift key selects a range of blobs.
Click with the control key adds a selection. Selected blobs are
highlighted graphically in the Image Viewer.
- How do I save the blob table?
Step 1: Select Save Blob Table from the File menu
of the Image Viewer.
GC Image launches a pop-up dialog.
Step 2: Enter the file name for the CSV .csv file, using
the file-system browser if desired, and indicate whether all blobs or
only included blobs are written to the file.
- How do I configure the blob table?
Step 1: Select Configure Blob Table from the Configure
menu of the Image Viewer.
GC Image launches the Configure Blob Table pop-up dialog.
Step 2: Select the desired features.
- Include desired feature(s) by click-and-pointing invisible feature(s),
using Shift-Click to select a range and control-click to select additional
features, and then clicking the add button.
- Un-include feature(s) by click-and-pointing visible feature(s) and
then clicking the remove button.
- The feature list can be ordered by selecting the location for changed
features on the destination list.
If desired, save the blob table configuration as the default.
Then click the OK button.
- How do I create a blob set?
Step 1: Create a graphic object.
Step 2: Set the inclusion flag for the graphic object. For
point and polyline graphics, the blob set is defined by intersection
of the graphic with blobs. For a rectangle and polygon graphics,
the blob set is defined by containment of the blob peaks by the graphic.
- How do I view the blob set table?
Step 1: Select Blob Set Table from the View menu
of the Image Viewer or click the Blob Set Table button
on the Image Viewer tool bar.
GC Image launches the Blob Set Table viewer.
Step 2: Use the scroll bars to navigate the blob set table.
Sort the table by clicking on a column header (and click again
to reverse order).
- How do I select blobs from the blob set table?
Step 1: Bring up the blob set table.
Step 2: Click on a row of the blob set table selects the blobs in
the blob set. Click and click again with the shift key selects a range
of blobs. Click with the control key adds a selection. Selected blobs
are highlighted graphically in the Image Viewer.
- How do I save the blob set table?
Step 1: Select Save Blob Set Table from the File
menu of the Image Viewer. GC Image launches a pop-up dialog.
Step 2: Enter the file name for the CSV .csv file, using
the file-system browser if desired.
- How do I find/select blobs by blob ID, compound name, group name, or constellation name?
Step 1: Select Find Blobs from the Edit menu of the
Image Viewer.
GC Image launches a pop-up dialog.
Step 2: Select the desired criteria. Then click the OK button.
- How do I perform quantitative selection or deselection of blobs?
Step 1: Select Filter Blobs from the Filter menu
of the Image Viewer.
GC Image launches a pop-up dialog.
Step 2: Select the desired area, volume, peak value, and volume
ratio values to filter blobs, then indicate filtering for selection or
deselection.
- How do I visualize blob statistics?
Step 1: Optionally, select one or more blobs. (Otherwise, all
blobs are visualized.)
Step 2: Select Visualize Blobs from the View menu
of the Image Viewer.
GC Image launches a tool for graphing bi-variate relationships for blob
statistics.
Step 3: Select the desired blob statistic for each axis.
- How do I use blob statistics visualizations for analysis?
Step 1: Perform Visualize Blobs.
Step 2: Designate a set of points in the graph by drawing a polygon
around them - clicking at each vertex and double-clicking at the final
vertex.
Step 3: Click the Assign Properties button to bring
up a popup for setting the selected blobs metadata.
Step 4: Set the properties of the designated blobs and click
the OK button.
Answers to questions about pattern recognition in GC Image.
- What are templates?
Templates are patterns of peaks and graphic objects observed in
previous image(s) used to recognize similar patterns of blobs in
subsequent image(s). Once recognized, the metadata (including
chemical name, group name, and internal standard association) for
the blobs in the target image can be set from the template.
GC Image has tools for creating and using templates.
The selectable template cursor modes are:
- Select template object - Select a graphic.
- Draw template point - Draw a template point.
- Draw template polyline - Draw a template polyline.
- Draw template rectangle - Draw a template rectangle.
- Draw template polygon - Draw a template polygon.
- Draw template peak - Draw a template peak (as a point).
- How do I add selected blob peaks to a template?
Step 1: Select blob(s), with metadata set as desired, to be
included in the template.
Step 2: Click the Add to template button.
- How do I add selected graphics to a template?
Step 1: Select graphic(s), with metadata set as desired, to be
included in the template.
Step 2: Click the Add to template button.
- How do I draw and edit peaks and graphic objects in a template?
Step 1: Set the cursor mode to the desired draw template
object mode (point, polyline, rectangle, polygon, or peak) from the
template cursor mode selector on the Image Viewer palette.
Step 2: Draw the desired template object using the same operations
as for graphic objects. (Template peaks are drawn at designated locations,
just as points are drawn.) After drawing, GC Image will revert to select
a template object mode with the drawn template object selected.
Step 3: Edit the position and shape of the template object using
the same operations as for graphic objects.
- How do I configure template display settings?
Step 1: Select Configure Settings from the Configure
menu of the Image Viewer.
GC Image launches a pop-up with various configuration dialog panes.
Step 2: Select the Template Display configuration pane.
Step 3: Set the color for the various classes of objects (included,
unflagged, excluded, internal standards, and internal standard association
lines) and the visibility for internal standard association lines, then
click the OK button.
- How do I select template object(s)?
Step 1: Set the cursor mode to select a template object mode
from the template cursor mode selector.
Step 2: Click on the existing template object to be selected.
Select additional template objects for the selection set by holding
the control key during the click of the left mouse-button while the
cursor is on the next template object to be selected. GC Image will
place handles on the selected template objects(s).
- How do I select all template objects?
Step 1: Set the cursor mode to select a template object mode
from the template cursor mode selector.
Step 2: Select Select All item from the Edit menu.
GC Image will select all template objects.
- How do I set and edit template object(s) metadata?
Step 1: Select one or more template peaks or one or more template
graphics using the select template object cursor mode. (Metadata for
template peaks and template graphics cannot be edited together.)
Step 2: Click the right mouse button. Depending on how many
template peaks or template graphics are selected, GC Image launches
either a pop-up metadata dialog for a single object or a pop-up metadata
dialog for multiple objects. The metata dialogs for template graphic
objects are the same as for graphic objects. The metadata dialogs for
template peaks are shown below.
Step 3: Enter the metadata settings, then click OK.
- How do I delete template object(s)?
Step 1: Select the existing template object(s) to be deleted.
Step 2: Click the Delete / Exclude Object button.
Confirmation is required.
- How do I save a template?
Step 1: Select Save Template from the File menu of
the Image Viewer.
GC Image launches a file-system brower to specify the destination file.
Step 2: Specify the destination filename, then click the
Save button.
- How do I load a template?
Step 1: Select the Load Template option from the
Filter menu or click the Load Template button.
GC Image presents a dialog that allows the user to browse template
files with a graphical overlay on the image before loading (i.e.,
without modifying the image template).
Step 2: Browse and select the desired template, specify whether
the existing template is to be overwritten or appended, then click
Load. Template peaks and graphics must be unique, so any
conflicting objects will not be appended and a message will be displayed
in a popup.
- How do I match a template to blobs in the image?
Step 1: Perform blob detection and create or load a template.
Step 2: Select Match Template from the File menu
of the Image Viewer or click the Match Template button on
the Image Viewer tool bar. GC Image graphically highlights
peak-to-blob matches. Template peak matches are shown as color-filled
circles at the matched location. If not all peaks are matched, a popup
reports the matching failures. Note that Match Template does
not change the image or the template, it merely graphically visualizes
the matches.
- How do I apply a matched template to blobs in the image?
Step 1: Perform Match Template.
Step 2: Select Apply Template from the File menu
of the Image Viewer or click the Apply Template button on
the Image Viewer tool bar.
- How do I configure template matching?
Step 1: Select Configure Settings from the Configure
menu of the Image Viewer.
GC Image launches a pop-up with various configuration dialog panes.
Step 2: Select the Template Matching configuration pane.
Step 3: Set the search window for translation and scale of the
template and the maximum distance (i.e., threshold) for matching
between a template peak and blob peak. Click the OK button.
Answers to questions about configuration files and operations journals.
- What is a configuration file?
GC Image provides a means of storing and recalling all the many
configuration settings for processing and analyzing images, the
color map, and the blob table configuration in a processing configuration
file.
- How do I export (i.e., save) a configuration file?
Step 1: Choose Configure Settings from the Configure
menu of the Image Viewer. This presents a configuration pane.
Step 2: Click the Export Configuration button on any of
the configuration panes. This presents a popup dialog.
Step 3: Select the filename and indicate whether the blob table
configuration and/or the color map are to be included.
- How do I import (i.e., load) a configuration file?
Step 1: Choose Configure Settings from the Configure
menu of the Image Viewer. This presents a configuration pane.
Step 2: Click the Import Configuration button on any of
the configuration panes. This presents a file system browser to select
a configuration file.
Step 3: Select the configuration file to be imported (i.e., loaded).
- What is an operations journal?
An operations journal is a record of the operations that have been
performed on an image. Each entry in the journal includes the
name of the operation, the parameters that were used in applying the
operation, and all information that is required to undo the operation.
The operations journal supports three operations:
- View the list of operations.
- Undo the most recent operation.
- Report the journal.
- How do I view the list of operations that have been performed on an image?
Step 1: Select the List Operations option from the
Edit menu of the Image Viewer. GC Image presents a
popup with a text box listing a brief description for each operation
that has been performed on the image.
Step 2: Close the List Operations popup by clicking
the OK button.
- How do I undo the most recent operation performed on an image?
Step 1: Click the Undo button or select the Undo
option from the Edit menu of the Image Viewer. The last
operation that was performed to modify the image will be reversed.
- How do I generate a formatted report of the operations journal?
Step 1: Select the List Operations option from the
Edit menu of the Image Viewer. GC Image presents a
List Operations popup.
Step 2: Click the View Journal button on the List
Operations popup. The user is prompted to name the destination
file for the report.
Step 3: Enter the destination file name for the report, then
click Save.
Answers to questions about GCxGC-MS data in GC Image.
- What GCxGC-MS tools are available in GC Image?
GC Image provides GCxGC-MS tools for:
- input and output;
- viewing mass spectra of samples, peaks, and blobs;
- selecting mass/z subranges of mass spectra;
- generating ion chromatograms;
- constructing and applying selection expressions;
- searching mass spectral libraries; and
- using mass spectral library searches for blob identification;
- What are the file structures for GCxGC-MS data?
GC Image stores GCxGC-MS data in two files:
-
a GCI file containing metadata (as described in chapter
Image Input and Output) and
-
a binary file containing the data in the Network Common Data Format
(NetCDF).
- How do I import GCxGC-MS data?
GC Image supports importing GCxGC-MS data from files in CDF. To import
GCxGC-MS data contained in a CDF file. Follow the same steps as for
importing GCxGC data.
- How do I perform input and output of GCxGC-MS data?
Using the GCI and CDF files, the GC Image file operations Open,
Recent Images, Save, Save As, Close, and
Exit work for GCxGC-MS images as they do for GCxGC images
(chapter Image Input and Output).
- How do I view a mass spectrum at a point?
Step 1: Select Point Mass Spectrum mode on the Image
Viewer palette.
Step 2: Click on a point in the total ion count (TIC) image.
- How do I view a mass spectrum at a blob peak?
Step 1: Perform blob detection.
Step 2: Select Peak Mass Spectrum mode on the Image
Viewer palette.
Step 3: Click within a blob in the total ion count (TIC) image.
- How do I view a mass spectrum for a blob?
Step 1: Perform blob detection.
Step 2: Select Blob Mass Spectrum mode on the Image
Viewer palette.
Step 3: Click within a blob in the total ion count (TIC) image.
- How do I select mass/z subranges of a mass spectrum?
Step 1: Display a point, peak, or blob mass spectrum.
Step 2: Within the graph area of the Mass Spectrum Viewer,
click-and-drag the left mouse-button to designate the desired subrange.
Step 3: If desired, change the subrange by repeating Step 2 or
add additional subranges by repeating Step 2 with the control key
depressed.
- How do I generate an ion chromatogram image?
Step 1: Select a mass/z subrange.
Step 2: Click the Ion Chromatogram button on the
Mass Spectrum Viewer.
- How do I configure mass spectral and ion chromatogram display?
Step 1: Select Configure Settings from the Configure
menu of the Image Viewer.
GC Image launches a pop-up with various configuration dialog panes.
Step 2: Select the Multi-Channel configuration pane.
Step 3: Select display for mass spectra in a single window or
multiple windows and display for spectal images in a single window or
multiple windows.
- How do I construct and apply selection expressions?
Step 1: Click the Select Expression button or choose the
Select Expression item from the View menu. GC Image presents
the Selection Tool.
Step 2: Build or load a selection expression.
Step 3: Click Select Blobs to select blobs satisfying the
expression or click Generate Selection Image to generate an image
masked to reveal only pixels satisfying the expression.
- How do I search a mass spectral library?
Step 1: Display a point, peak, or blob mass spectrum. If desired,
select one or more mass/z subranges.
Step 2: Click the Search MS Library button on the Mass
Spectrum Viewer.
- How do I configure mass spectral searching?
Step 1: Select Configure Settings from the Configure
menu of the Image Viewer.
GC Image launches a pop-up with various configuration dialog panes.
Step 2: Select the Search MS Library configuration pane.
- How do I use mass spectral library searches for blob identification?
Step 1: Perform blob detection.
Step 2: Click the Search MS Library button on the Image
Viewer tool bar or select Search MS Library from the Filter
menu.
- How do I correct blob mis-identifications made by mass spectral library search?
Step 1: Select the mis-identified blob using the select blobs cursor
mode.
Step 2: Click the right mouse button. GC Image launches a pop-up
metadata dialog for a single blob.
Step 3: Click the Hit List button. GC Image launches a
hit-list popup.
Step 4: Click Select flag for the correct chemical
identification, then click OK.
Questions about project management and reporting in GC Project.
- What tools are available in GC Project for project management and reporting?
GC Project provides support for organizing and reporting on the
sets of data developed during GCxGC analyses.
GC Project is invoked from the desktop icon, from the Microsoft
Windows Programs menu, or from the installation directory.
The GC Project graphical user-interface (GUI) has a title bar,
menu bar, tool bar, project tree, and component information pane. The
lower-left pane contains the Project Tree that visualizes and
provides access to the project components. The lower-right pane presents
Component Information about the user-selected component(s) and
provides access to component operations.
A node in the project tree can be selected by clicking on it. This
causes information about the node and buttons for relevant operations
to be displayed in the Component Information pane. Some nodes
(e.g., image nodes) have actions initiated by double-clicking
(e.g., opening the image with GC Image).
In the Project Tree, if a node has a '+', then it can be expanded
by clicking the '+' to list the children in the tree. If a node has a '-',
then it can be collapsed by clicking the '-' so that the list of children
is not shown. If there is neither a '+' nor '-', then there are no
children of the node.
- What is a project?
A project consists of a directory named for the project, a GC
Project file named for the project with extension .gcp
in the project directory, and sub-directories:
-
Vials for composition files,
-
Runs for raw chromatographic data,
-
Processing for processing files,
-
Images for processed image files, and
-
Reports for report files.
- What is a vial in a project?
Vials are elements that describe the different mixtures analyzed in
the project.
- What is a run in a project?
Runs are unprocessed chromatograms.
- What is a processing file in a project?
Processing files are files used for processing chromatograms into
images, including configuration files, blob templates, and graphics
files.
- What is a processed image in a project?
Processed images are the results of processing and analyzing raw
chromatograms using GC Image.
- What is a batch in a project?
Batches are sets of processed images that are conveniently grouped
for reporting.
- What is a report in a project?
A report is a formal presentation of information about processed images.
Reports are formatted as XML (preferred), HTML, or CSV.
Questions about project files and directories in GC Project.
- How do I start GC Project?
Step 1: Execute the GC Project desktop icon, or choose
GC Project from the Microsoft Windows Programs menu, or execute
GC Project from the installation directory.
- How do I create a new project?
Step 1: Click the New Project button on the tool bar or
select New Project from the File menu of GC Project.
Step 2: Specify the directory location and the project name.
- How do I save a project?
Step 1: Click the Save Project button on the tool bar or
select Save Project from the File menu of GC Project.
- How do I open an existing project?
Step 1: Click the Open Project button on the tool bar or
select Open Project from the File menu of GC Project.
Step 2: Specify the GC Project (extension .gcp) file
in the file browser.
- How do I close a project?
Step 1: Click the Close Project button on the tool bar or
select Close Project from the File menu of GC Project.
Step 2: If the project has been modified since the most recent
save operation, indicate whether or not the project should be saved.
- How do I exit GC Project?
Step 1: Select Exit from the File menu of GC
Project.
- How do I import composition tables into a project?
Step 1: Create or copy a CSV file containing the composition
table for each vial into the Vials subdirectory (under the
project directory). The format is text with each line
"<compound-name>,<amount>". Compound names containing
commas should be enclosed in double quotation marks.
- How do I import raw chromatographic data into a project?
Step 1: Copy raw chromatogram files (e.g., CSV format) for each run
into the Runs subdirectory (under the project directory). The file
format must be readable by GC Image.
- How do I import processing files into a project?
Step 1: Copy GC Image processing files (e.g., configuration
files, blob templates, and graphics files) into the Processing
subdirectory (under the project directory).
Questions about vials in GC Project.
- How do I view the list of vials in a project?
Step 1: Click the Vials node in the Project Tree.
- How do I create vials in a project?
Step 1: Click the Vials node in the Project Tree.
Step 2: Click the Add Vial button in the Component
Information pane.
Step 3: Specify the root-name of the vial, a starting sequence
number for the vial, the number of vials to be created (with the sequence
number incremented for each new vial), and vial metadata. Click the
OK button.
Step 4: If there is a CSV-format composition table in the
Vials directory with the same name as the vial, confirm or
deny importation of the table.
- How do I view information about a vial in a project?
Step 1: Expand the Vials node in the Project Tree.
Step 2: Select a Vial node in the Project Tree.
- How do I import a composition table for a vial?
Step 1: View a vial in the Component Information pane.
Step 2: Click the Import Composition button.
Step 3: Specify the composition file (CSV format) in the file browser.
- How do I add a record to a composition table for a vial?
Step 1: View a vial in the Component Information pane.
Step 2: Click the Add Record button.
Step 3: Enter the compound name and amount, then click the OK
button.
Questions about runs in GC Project.
Questions about processing files in GC Project.
Questions about processed images in GC Project.
Questions about the hierarchical overview in GC Project.
Questions about batches in GC Project.
Questions about reports in GC Project.
Contents
GC Image™ Users' Guide © 2001–2004 by GC Image, LLC, and the University of Nebraska.
