Juan Cui

Assistant Professor, Department of Computer Science and Engineering
Courtesy Assistant Professor, School of Biological Science
Univeristy of Nebraska-Lincoln
Courtesy Assistant Professor, Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center

122B, Avery Hall, 1144 T Street
Lincoln, NE, 65888-0115
Phone: 402-472-5023
Email: jcui@cse.unl.edu
Visit Cui' Group (Most Updated) >>>

Research Interests:

Computational and systems biology, bioinformatics, cancer informatics, data mining and machine learning


Education and Training:

•  Assistant research scientist/group leader in cancer bioinformatics (2011~2013), Department of Biochemistry and Molecular Biology, University of Georgia

•  Postdoc. /group leader in cancer bioinformatics (2008~2011), Department of Biochemistry and Molecular Biology, University of Georgia

•  Ph.D in Bioinformatics (2008), Department of Computational Science and Department of Pharmacy, National University of Singapore

•  B.E. in Information engineering (2002), School of Electronic Engineering, Northwestern Polytechnic University, China


Journal Publications: (Check the most recent update >>>)

  1. Chiang K, Shu J, Zempleni J, Cui J. Dietary MicroRNA Database (DMD): An archive database and analytic tool for microRNAs in human foods. PLoS ONE. 2015 Accepted.
  2. Zempleni J, Baier SR, Howard KM, Cui J. Gene regulation by dietary microRNAs. Invited paper, Can J Physiol Pharmacol, April 2015, 10.1139/cjpp-2014-0392.
  3. Juan Cui, Yanbin Yin, Qin Ma, Guoqing Wang, Victor Olman, Yu Zhang, Wen-Chi Chou, Celine S. Hong, Chi Zhang, Sha Cao, Xizheng Mao, Ying Li, Steve Qin, Shaying Zhao, Jing Jiang, Phil Hastings, Fan Li, Ying Xu. Comprehensive Characterization of Genomic Alterations in Human Gastric Cancer International Journal of Cancer 2014 Nov 24. doi: 10.1002/ijc.29352.
  4. Beach S, Brody G, Lei GH, Kim S, Cui J, Philibert R. Is serotonin transporter genotype associated with epigenetic susceptibility or vulnerability? Examination of the impact of socioeconomic status risk on African American youth Development and Psychopathology 2014, 26:289–304
  5. Qin Ma, Jaxk H. Reeves, David A. Liberles, Lili Yu, Zheng Chang, Jing Zhao, Juan Cui, Ying Xu, Liang Liu. A phylogenetic model for understanding the effect of gene duplication on cancer progression Nucleic Acids Research 2014, doi: 10.1093/nar/gkt1320
  6. Sun H, Wang HP, Tang KL, Qin G, Cui J, Cao ZW, Liu Qi. iPEAP: integrating multiple omics and genetic data for pathway enrichment analysis, Bioinformatics  2013 Oct 30. doi: 10.1093 [Epub ahead of print]
  7. Jiaxin Wang, Yanchun Liang, Yan Wang, Juan Cui, Ming Liu, Wei Du, Ying Xu, Computational Prediction of Human Salivary Proteins from Blood Circulation and Application to Diagnostic Biomarker Identification, submitted to PLoS ONE, 2013 Nov 12;8(11): e80211
  8. Dong XY, Wang GQ, Zhang GQ, Ni ZH, Suo J, Cui J, Cui A, Xu Y, Li F (2013) The endothelial lipase protein is promising urinary biomarker for diagnosis of gastric cancer. Diagnostic Pathology 8 (1), 45, 2013.
  9. Xu K, Mao XZ, Mehta M, Cui J, Zhang C, Mao FL, Xu Y (2013) Elucidation of How Cancer Cells Avoid Acidosis through Comparative Transcriptomic Data Analysis, PLoS ONE, e71177, 2013.
  10. Cui J, Mao X, Olman V, Hastings PJ, Xu Y (2012) Hypoxia and miscoupling between reduced energy efficiency and signaling to cell proliferation drive cancer to grow increasingly faster. Journal of Molecular Cell Biology 4: 174-176.
  11. Cui J, Liang YC, Xu Y (2012) Systems biology in the frontier of cancer research: a report on the Second International Workshop of Cancer Systems Biology. Chin J Cancer. Aug 28. doi: 10.5732/cjc.012.10205.
  12. Su Y, Ni Z, Wang G, Cui J, Wei C, et al. (2012) Aberrant expression of microRNAs in gastric cancer and biological significance of miR-574-3p. International Immunopharmacology 13: 468-475.
  13. Liu Q, Zhou H, Cui J, Cao Z, Xu Y (2012) Reconsideration of in-silico siRNA design based on feature selection: a cross-platform data integration perspective. PLoS ONE 7: e37879.
  14. Xu K, Mao X, Mehta M, Cui J, Zhang C, et al. (2012) A comparative study of gene-expression data of basal cell carcinoma and melanoma reveals new insights about the two cancers. PLoS ONE 7: e30750.
  15. Cui J, Eldredge JB, Xu Y, Puett D (2011) MicroRNA expression and regulation in human ovarian carcinoma cells by luteinizing hormone. PLoS ONE 6.: e21730
  16. Cui J, Miner BM, Eldredge JB, Warrenfeltz SW, Dam P, et al. (2011) Regulation of gene expression in ovarian cancer cells by luteinizing hormone receptor expression and activation. BMC Cancer 11: 280.
  17. Cui J, Chen Y, Chou WC, Sun L, Chen L, et al. (2011) An integrated transcriptomic and computational analysis for biomarker identification in gastric cancer. Nucleic Acids Research 39: 1197-1207.
  18. Cui J, Li F, Wang G, Fang X, Puett JD, et al. (2011) Gene-expression signatures can distinguish gastric cancer grades and stages. PLoS ONE 6: e17819.
  19. Gao J*, Cui J*, Wang Y, Dong ZN, Tian YP, et al. (2011) Identification of potential predictors for subtype IgA nephropathy through analyses of blood biochemical indicators. Clinica Chimica Acta 412: 441-445.
  20. Hong CS, Cui J, Ni Z, Su Y, Puett D, et al. (2011) A computational method for prediction of excretory proteins and application to identification of gastric cancer markers in urine. PLoS ONE 6: e16875.
  21. Xu K*, Cui J*, Olman V, Yang Q, Puett D, et al. (2010) A comparative analysis of gene-expression data of multiple cancer types. PLoS ONE 5: e13696.
  22. Zhang X, Cui J, Nilsson D, Gunasekera K, Chanfon A, et al. (2010) The Trypanosoma brucei MitoCarta and its regulation and splicing pattern during development. Nucleic Acids Research 38: 7378-7387.
  23. Liu Q, Cui J, Yang Q, Xu Y (2010) In-silico prediction of blood-secretory human proteins using a ranking algorithm. BMC Bioinformatics 11: 250.
  24. Lu Y, Gao Y, Cao Z, Cui J, Dong Z, et al. (2010) A study of health effects of long-distance ocean voyages on seamen using a data classification approach. BMC Med Inform Decis Mak 10: 13.
  25. Diao H, Xiao S, Cui J, Chun J, Xu Y, et al. (2010) Progesterone receptor-mediated up-regulation of transthyretin in preimplantation mouse uterus. Fertility and Sterility 93: 2750-2753.
  26. Jia J, Cui J, Liu X, Han J, Yang S, et al. (2009) Genome-scale search of tumor-specific antigens by collective analysis of mutations, expressions and T-cell recognition. Mol Immunol 46: 1824-1829.
  27. Cui J, Liu Q, Puett D, Xu Y (2008) Computational prediction of human proteins that can be secreted into the bloodstream. Bioinformatics 24: 2370-2375.
  28. Cui J, Han LY, Lin H, Tang ZQ, Ji Z, et al. (2007) Advances in exploration of machine learning methods for predicting functional class and interaction profiles of proteins and peptides irrespective of sequence homology. Current Bioinformatics 2: 95-112.
  29. Tang ZQ, Han LY, Lin HH, Cui J, Jia J, et al. (2007) Derivation of stable microarray cancer-differentiating signatures using consensus scoring of multiple random sampling and gene-ranking consistency evaluation. Cancer Res 67: 9996-10003.
  30. Cui J, Han LY, Lin HH, Zhang HL, Tang ZQ, et al. (2007) Prediction of MHC-binding peptides of flexible lengths from sequence-derived structural and physicochemical properties. Mol Immunol 44: 866-877.
  31. Cui J, Han LY, Li H, Ung CY, Tang ZQ, et al. (2007) Computer prediction of allergen proteins from sequence-derived protein structural and physicochemical properties. Mol Immunol 44: 514-520.
  32. Xie B, Zheng CJ, Han LY, Ong S, Cui J, et al. (2007) PharmGED: pharmacogenetic effect database. Clin Pharmacol Ther 81: 29.
  33. Zheng CJ, Han LY, Xie B, Liew CY, Ong S, et al. (2007) PharmGED: Pharmacogenetic Effect Database. Nucleic Acids Research 35: D794-799.
  34. Cui J, Han LY, Lin HH, Tang ZQ, Jiang L, et al. (2006) MHC-BPS: MHC-binder prediction server for identifying peptides of flexible lengths from sequence-derived physicochemical properties. Immunogenetics 58: 607-613.
  35. Han L, Cui J, Lin H, Ji Z, Cao Z, et al. (2006) Recent progresses in the application of machine learning approach for predicting protein functional class independent of sequence similarity. Proteomics 6: 4023-4037.
  36. Zheng CJ, Han LY, Chen X, Cao ZW, Cui J, et al. (2006) Information of ADME-associated proteins and potential application for pharmacogenetic prediction of drug responses. Current Pharmacogenomics 4: 87-103.
  37. Cui J, Han LY, Cai CZ, Zheng CJ, Ji ZL, et al. (2005) Prediction of functional class of novel bacterial proteins without the use of sequence similarity by a statistical learning method. J Mol Microbiol Biotechnol 9: 86-100.
  38. Han LY, Zheng CJ, Lin HH, Cui J, Li H, et al. (2005) Prediction of functional class of novel plant proteins by a statistical learning method. New Phytol 168: 109-121.
  39. Han LY, Cai CZ, Ji ZL, Cao ZW, Cui J, et al. (2004) Predicting functional family of novel enzymes irrespective of sequence similarity: a statistical learning approach. Nucleic Acids Research 32: 6437-6444.


  1. Xu Y, Cui J and J. Dave Puett, (Introduction to Cancer Bioinformatics) , Springer, New York.

Invited Chapters: 

  1. Cui J Cui J. “Genomic Data Analysis for Personalized Healthcare”. In Healthcare Data Analytics. Edited by Chandan K. Reddy, Charu C. Aggarwal. CRC Press (Data Mining and Knowledge Discovery Series), 2015.
  2. Cui J, Xu Y, Puett D. 2013. Microarray-Based Transcriptome Profiling of Ovarian Cancer Cells. In Ovarian cancer - Methods and Protocols. Springer, New York. doi:10.1007/978-1-62703-547-7
  3. Cui J, Xu Y, Puett D. 2012. Transcriptomic Analysis of Human Ovarian Cancer Cells: Changes Mediated by Luteinizing Hormone Receptor Activation. In Ovarian Cancer. InTech, Rijeka. doi:10.5772/28204

Conference Publications: 

  1. Shu J, Chiang K, Cui J. Computational characterization of microRNA-mediated association between obesity and cancer. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research(AACR); 2015 Apr 18-22; Philadelphia, PA.2015
  2. Zempleni J, Baier SR, Cui J, Wolf T. Bovine microRNAs are bioavailable and affect gene expression in humans and mice. American Society for Cell Biology, Philadelphia, PA, October 6-10, 2014
  3. Yan Wang, Guishen Wang, Di Meng, Lan Huang, Enrico Blanzieri, Juan Cui (2014) A Markov Clustering based Link Clustering Method for Overlapping Module Identification in Yeast Protein-protein Interaction Networks. THE 10th International Symposium on Bioinformatics Research and Applications (ISBRA2014), June 2014, Zhangjiajie, China
  4. Toward understanding the genomic alterations in human gastric cancer. Abstract in Proceedings of the American Association of Caner Research (AACR), April 2014, San Diego, US


Manuscripts Submitted or in Preparation:

  1. Shu J, Chiang K, Zempleni J, Cui J.Computational Characterization of Exogenous MicroRNAs That Can Be Transferred into Human Circulation. To be submitted to PLoS ONE 
  2. Chiang K, Shu J, Zempleni J, Cui J. Dietary MicroRNA Database (DMD): An archive database and analytic tool for microRNAs in human foods. To be submitted to PLoS ONE 
  3. Wang Y, Wang GS, Meng D, Huang L, Cui J, Blanzieri E. A Markov lustering based link clustering method for overlapping module identification in yeast PPI network. Submitted 2013
  4. Wang Y*, Cui J*, Du W, Zhang C, Mao FL, Wang JX, Chen L, Xu Y. Identification of highly-specific gene markers in gastric cancer through meta- transciptome anlaysis on multiple human diseases gastric gene markers with high specificity based on gene expression data of multiple human diseases. Submitted to Bioinformatics
  5. Hong CS, Cui J, Xu, Y. Characterization of urine-excretory miRNAs and demonstration of their Feasibility as disease diagnostic markers. Submitted to PLoS ONE
  6. Beach S, Brody G, Lei GH, Kim S, Cui J, Philibert R. The Effect of Early Socioeconomic-Related Stress on Epigenetic Reprogramming of Depression-Related Genes Mediates its Association with Depressive Symptoms in African American Young Adults. Submitted to Psychosomatic Medicine
  7. Chao Zuo, Chi Zhang, Juan Cui, Kun Xu and Ying Xu, Derivation of Common and Distinct Characteristics of Different Cancer Types through Comparative Transcriptomic Data Analyses, submitted, 2012
  8. Cui J, Wang Y, Xu Y. Cell growth and metabolism in cancer: new insights gained through comparative transcriptome analysis across different cancers.  In preparation


Invited Talks and Conference Posters:

•   Talk: International Cancer System Biology conference (ICSB), Changchun, China, 07/2015 

•   Talk: “Computational construction of microRNA regulation network in cancer”, UNMC Department of Biochemistry, 05/2015  

•  Talk: “Omics driven biomarker discovery and genome study in cancer”, Department of Biomedical Sciences, Creighton University School of Medicine, 11/2014  

•  Talk: Zempleni J, Baier SR, Cui J, Wolf T. Bovine microRNAs are bioavailable and affect gene expression in humans and mice. American Society for Cell Biology, Philadelphia, PA, October 6-10, 2014  

•  Talk: “Omics driven cancer research” in Nebraska Gateway to Nutrigenomics, UNL, 03/2014  

•  Talk: “Characterization of genome and metabolic network in gastric cancer” in UNMC, 12/2013  

•  Talk: “From Diagnostic Biomarker Discovery to Molecular Mechanism Study: a bioinformatics approach to gastric cancer research” in the School of Biological science, 09/2013  

•  Talk: “Comprehensive elucidation of genomics alterations in human gastric cancer” in Biostatistics at Emory University, 11/2012 

•  Talk: “Biomarker identification for cancer diagnosis” in the Winship Cancer Institute at Emory University, 08/2012

•  Talk: "Whole-genome sequence analysis reveals new insights about gastric cancer" in International Cancer System Biology conference, Changchun, China, 07/2012

•  Talk: "Bioinformatics in Cancer Research", Tongji University, China, 07/2012

•  Invited lectures:"Cancer informatics", Tallinn University and Tartu University, Estonia, 06/2012

•  Poster, "Biomarker identification for gastric cancer diagnosis" Cancer Detection and Diagnostics Technologies for Global Health Conference, NIH Campus, August/2011

•  Talk: "Biomarker identification for cancer diagnosis" in International Cancer System Biology conference, Changchun, China, 07/2011

•  Talk: "cancer marker identification" in Biostatistics department at Emory University, 03/2011

•  Talk: "Gene Expression Profiling of Ovarian Cancer Cells Post-Activation of the LH Receptor",Co-author, 14th World Congress of Gynecological Endocrinology, Florence, Italy, 03/2010

•  Poster: "An integrated approach to cancer marker identification" in GRA Vaccine Dinner Club at Emory University, GA, 02/2010

•  Invited Talk: "Biomarker Identification for Cancer" in CHI's meeting: Accelerating Development & Advancing Personalized Therapy (ADAPT), Washington, 09/2009

•  Talk: "A study of gastric cancer maker identification" in Department of Biomedicine Engineering, the Fourth Military Medical University, Xi'an, China 12/2009

•  Talk: "An integrated approach to cancer marker identification" in Department of Computer Science, Jilin University, Changchun, China 12/2009

•  Talk: "Identification of gene/protein signatures for gastric cancer diagnosis" in No. 2 Affiliated Hospital, Jilin University, Changchun, China 12/2009

•  Talk: "Gastric cancer biomarkers" in Department of Life Science, Northeastern Normal University, Changchun, China 11/2009

•  Poster: "Towards Early Detection of Human Cancer: a systems biology approach to identify serum biomarker for gastric cancer" in Computational System Biology (CSB), Stanford, 08/2009

•  Poster: "A computational program for blood secreted protein prediction: toward serum marker identification" in Computational System Biology (CSB), Stanford, 08/2008

•  Poster: "Computational Prediction of Human Proteins That Can Be Secreted into the Bloodstream" in Third Annual CSBS 2008 Symposium, UGA, 03/2008

•  Attendee. 3rd AOHUPO and 4th Structural Biology and Functional Genomics Conference, Singapore, 2006

•  Attendee. Workshop on Machine learning for Biomedical Informatics, Singapore, 2006

•  Attendee. APBC 2005 Graduate Student Satellite Symposium, Singapore, 2005

•  Attendee. The 5th HUGO Pacific Meeting, Singapore, 2004






Academic Services :

Associate Editor: BMC Research Notes (2010- present)

Editorial Board Member: Journal of Nutritional Biochemistry (2014-)

Editorial Board Member: Genomics, Proteomics & Bioinformatics (2012-)

Editorial Board Member: JSM Computer Science & Engineering (2013-)

Conference/workshop Organizer: 

Chair of UNL bioinformatics workshop on Microarray and NGS data analysis, 07/2014

Co-chair of the International Cancer System Biology conference, Changchun, China, 2011-2012

Chair of “Cancer Informatics” workshop at IEEE BIBM, Atlanta, 2011 

Organizing member of "Forest Tree Microarray Workshop" University of Georgia, 2009 

Ad-hoc Proposal Reviewer:  

Internal funding initiative INPHAASE (the Institute for Population Sciences, Health Assessment, Administration, Services, and Economics), Wayne State University, 2014

Program Committee Member: 

IEEE International Conference on Bioinformatics and Biomedicine (BIBM) 2008-2012 

IEEE Computational Intelligence in Bioinformatics and Computational Biology (CIBCB) 2012

International Symposium on Enabled Health Informatics, Bio-Medicine and Bioinformatics (HI-BI-BI) 2012

International Conference on Intelligent Biology and Medicine (ICIBM) 2012

IEEE 2nd International Conference on Computational Advances in Bio and Medical Sciences (ICCABS) 2012 

IEEE International Conference on Computational Advances in Bio and Medical Sciences (ICCABS) 2011

IEEE 12th International Conference on Bioinformatics & Bioengineering (BIBE) 2011

IEEE International Conference on Biomedical Engineering and Informatics (BMEI) 2011 

IEEE International Conference on Data Mining series (ICDM) 2010-2011 

International Conference in Foundations of Computer Science & Technology (FCST) 2010

International Bioinformatics workshop (IBW) 2010

International Conference on Machine Learning and Applications (ICMLA) 2008 

Reviewer for Journals: 

Amino Acids


BMC Bioinformatics

BMC Genomics

BMC Research Notes


Frontiers in Bioscience

International Journal of Data Mining and Bioinformatics

Journal of Bioinformatics and Computational Biology

Journal of Bioinformatics and System Biology

Journal of Biomedicine and Biotechnology

Mathematical Biosciences

Molecular and Cellular Endocrinology

Nucleic Acids Research


Tumor Biology

Reviewer for Conferences: 

        Computational Systems Bioinformatics Conference (CSB)2008-2009

        Asia Pacific Bioinformatics Conference (APBC) 2009

        ACM International Conference On Bioinformatics and Computational Biology(ACM-BCB) 2010

        International Conference on Intelligent Computing (ICIC) 2010-2011

        Computational Intelligence in Bioinformatics and Computational Biology (CIBCB) 2012 



Data structure and algorithms (CSCE311), Spring 2014-2015 

Computational methods in bioinformatics (CSCE496/896), Fall 2013-2014 



•  Nebraska Gateway to Nutrigenomics (NGN)

•  Interdepartmental Nutrition Program (INP), UNL

•  International Society for Computational Biology

•  Active member, American Association of Cancer Research